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Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes.

Identifieur interne : 001800 ( Main/Exploration ); précédent : 001799; suivant : 001801

Comparisons of envelope through 5B sequences of infectious bronchitis coronaviruses indicates recombination occurs in the envelope and membrane genes.

Auteurs : Judy Elaine Brooks [États-Unis] ; Aaron Cameron Rainer ; Rebecca Lynn Parr ; Peter Woolcock ; Fred Hoerr ; Ellen Whited Collisson

Source :

RBID : pubmed:15019237

Descripteurs français

English descriptors

Abstract

A 1.78 kb sequence, including the E, M, 5a and 5b genes, and the intergenic region between the M and 5a genes, of six US strains of infectious bronchitis (corona)virus (IBV) were sequenced and compared to the published sequences for two additional strains. The overall identities as determined through pairwise analyses of nucleotide sequences of the entire 1.78kb region ranged from 90 to 99%, with the 5b open reading frame (ORF) having the greatest identity (94-99%) while the identities of the E, 5a and M ORFs ranged from 87 to 100%. Nucleotide sequencing of recent field isolates from Alabama (Ala1) and California (Cal3) revealed distinct shifts in homology in the M gene, indicating two apparent recombination events between the Holland 52/Mass41-like strain and an Ark-like strain, both origins of commonly used vaccine strains. Putative sites of recombination could also be identified in both the E and M ORFs of laboratory strains of IBV.

DOI: 10.1016/j.virusres.2003.11.016
PubMed: 15019237


Affiliations:


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Le document en format XML

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<nlm:affiliation>Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station, TX 77843, USA.</nlm:affiliation>
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<term>Base Sequence</term>
<term>California</term>
<term>Chick Embryo</term>
<term>Conserved Sequence</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>DNA Primers</term>
<term>Infectious bronchitis virus (classification)</term>
<term>Infectious bronchitis virus (genetics)</term>
<term>Infectious bronchitis virus (isolation & purification)</term>
<term>Membrane Proteins (chemistry)</term>
<term>Membrane Proteins (genetics)</term>
<term>Molecular Sequence Data</term>
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<term>Sequence Homology, Nucleic Acid</term>
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<term>Cadres ouverts de lecture</term>
<term>Californie</term>
<term>Données de séquences moléculaires</term>
<term>Embryon de poulet</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Maladies de la volaille (virologie)</term>
<term>Phylogénie</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines membranaires ()</term>
<term>Protéines membranaires (génétique)</term>
<term>Protéines virales ()</term>
<term>Protéines virales (génétique)</term>
<term>RT-PCR</term>
<term>Recombinaison génétique</term>
<term>Réaction de polymérisation en chaîne</term>
<term>Similitude de séquences d'acides nucléiques</term>
<term>Séquence conservée</term>
<term>Séquence nucléotidique</term>
<term>Virus de la bronchite infectieuse ()</term>
<term>Virus de la bronchite infectieuse (génétique)</term>
<term>Virus de la bronchite infectieuse (isolement et purification)</term>
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<term>Viral Envelope Proteins</term>
<term>Viral Proteins</term>
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<term>Membrane Proteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Proteins</term>
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<term>DNA Primers</term>
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<term>Poultry Diseases</term>
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<term>Base Sequence</term>
<term>Chick Embryo</term>
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<term>Molecular Sequence Data</term>
<term>Open Reading Frames</term>
<term>Phylogeny</term>
<term>Polymerase Chain Reaction</term>
<term>Recombination, Genetic</term>
<term>Reverse Transcriptase Polymerase Chain Reaction</term>
<term>Sequence Alignment</term>
<term>Sequence Homology, Nucleic Acid</term>
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<term>Alignement de séquences</term>
<term>Amorces ADN</term>
<term>Animaux</term>
<term>Cadres ouverts de lecture</term>
<term>Californie</term>
<term>Données de séquences moléculaires</term>
<term>Embryon de poulet</term>
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<term>Recombinaison génétique</term>
<term>Réaction de polymérisation en chaîne</term>
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<front>
<div type="abstract" xml:lang="en">A 1.78 kb sequence, including the E, M, 5a and 5b genes, and the intergenic region between the M and 5a genes, of six US strains of infectious bronchitis (corona)virus (IBV) were sequenced and compared to the published sequences for two additional strains. The overall identities as determined through pairwise analyses of nucleotide sequences of the entire 1.78kb region ranged from 90 to 99%, with the 5b open reading frame (ORF) having the greatest identity (94-99%) while the identities of the E, 5a and M ORFs ranged from 87 to 100%. Nucleotide sequencing of recent field isolates from Alabama (Ala1) and California (Cal3) revealed distinct shifts in homology in the M gene, indicating two apparent recombination events between the Holland 52/Mass41-like strain and an Ark-like strain, both origins of commonly used vaccine strains. Putative sites of recombination could also be identified in both the E and M ORFs of laboratory strains of IBV.</div>
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